Cleavage Under Targets & Tagmentation ( CUT&Tag) is epigenomic profile method in which the Tn5 transposase is loaded with adaptor DNA. Tethering Tn5 to DNA-associated proteins allows concurrent cleavage and tagging of DNA adjacent to targeted chromatin elements in a single step. PCR enrichment and NGS sequences of this “Tagmented” DNA yields high-resolution genome-wide chromatin profiles. Further, the Henikoff research group has generated Cleavage Under Targeted Accessible Chromatin (CUTAC), which is a modification of CUT&Tag to produce chromatin accessibility maps by altering key parameters within the method. Used in parallel, CUT&Tag and CUTAC efficiently map chromatin profiles and the geography of open chromatin, active regulatory elements, and DNA bound transcription factors, with resolution and sensitivity that mirrors ATAC-seq and other more time intensive, complex, and laborious methods.
- In situ genome-wide mapping of DNA-protein interactions
- High resolution profiling of DNA regulatory elements and chromatin architecture
- Unifying analysis of both chromatin accessibility and epigenetic landscape simultaneously
- Single cell analysis of chromatin features, including rare cell types
- Simple, rapid, and cost-effective procedure done in a single tube, completed within a day
- Multiplex-ready and compatible with low solubility complexes
- High signal-to-noise ratio greatly reduces starting material and sequencing depth requirements
- PCR enriched libraries easily integrate with single cell sequencing platforms on the market
- High-throughput ready workflows for in-depth epigenetic analysis
Patent Pending, Publication number WO/2019/060907
In 2017 the market for epigenetic profiling was valued at $5.25 billion USD and is expected to grow to $22.05 billion by 2025 with a CAGR of 19.7%1 Kits and product reagents are key market drivers with ChIP-seq and ATAC-seq being the closest marketed competitors to CUT&Tag and CUTAC, respectively. The key advantages and new capabilities of CUT&Tag and CUTAC are clear improvements over prior technology making CUT&Tag and CUTAC suitable for widespread adoption.
- Steven Henikoff, PhD; Basic Sciences Division