Dr. Fredricks has developed a novel diagnostic method for detecting and characterizing fungal pathogens in patients. Although fungal spores are frequently inhaled by healthy humans, immunocompromised patients are susceptible to these pathogens causing life-threatening infections, and so rapid and accurate identification of fungal pathogens can be critical for initiating treatment in the earliest stages of infection. This method involves isolation of patient samples followed by PCR reactions to generate an amplicons in the fungal ITS2 region and the 28S ribosomal RNA genes. This method provides a distinct advantage to culture-based diagnosis, using effective lysis of fungal cell walls coupled with the ability to distinguish among fungal species. This technology also encompasses the detection of invasive pulmonary aspergillosis (IPA) with an optimized qPCR assay platform. IPA has a high mortality despite appropriate antifungal therapy, and so early detection and treatment is key.
- Respiratory infections
- Invasive pulmonary aspergillosis
- Immunocompromised patients
- Efficient method for fungal detection
- Ability to distinguish between fungal species
- Invasive pulmonary aspergillosis detection
- US 8450059
- US 20100129821
- EP 2370811
Millions of people around the world are diagnosed with a fungal pathogen infection every year. Immunocompromised patients are particularly susceptible to fungal infections with incidence of up to 9% of immunocompromised patients leading to a high mortality rate.
David Fredricks, MD, Vaccine and Infectious Disease Division